Characterization of human rotavirus genotype P[8]G5 from Brazil by probe-hybridization and sequence

@article{Alfieri1996CharacterizationOH,
  title={Characterization of human rotavirus genotype P[8]G5 from Brazil by probe-hybridization and sequence},
  author={Alice Fernandes Alfieri and Jos{\'e} Leite and Osamu Nakagomi and Akikazu Kaga and Phyllis A Woods and RogerI. Glass and Jon R. Gentsch},
  journal={Archives of Virology},
  year={1996},
  volume={141},
  pages={2353-2364}
}
We report the molecular characterization of rotavirus genotype P[8]G5 strains found in fecal specimens collected in four different regions of Brazil, using digoxigenin (dig)-labeled oligonucleotide probes, sequence analysis, and RNA-RNA hybridization. The closest sequence relationships of the neutralization antigens of these strains were to the VP4 protein of P1A[8]G1 strain KU (93.3% identity in amino acids 11 to 282) and to the VP7 protein of G serotype 5 strain OSU (87.6% identity in amino… CONTINUE READING
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