Characterization of human liver microsomal UDP-glycosyltransferases using photoaffinity analogs.

@article{Little1995CharacterizationOH,
  title={Characterization of human liver microsomal UDP-glycosyltransferases using photoaffinity analogs.},
  author={Joanna M. Little and Richard R. Drake and Roel J Vonk and Folkert Kuipers and Roger Lester and Anna Radominska},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1995},
  volume={273 3},
  pages={1551-9}
}
The photoaffinity analogs [beta-32P]5-azido-UDP-glucuronic acid ([32P]5N3UDP-GlcUA) and [beta-32P]5-azido-UDP-glucose ([32P]5N3UDP-Glc) were used to characterize UDP-glycosyltransferases of microsomes prepared from human liver. Photoincorporation of both probes into proteins in the 50- to 56-kdalton range, known to contain UDP-glucuronosyl transferases (UGTs), was concentration dependent, and photolabeled proteins were susceptible to trypsin digestion only in the presence of detergent. The… CONTINUE READING