Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

  title={Characterization of human embryonic stem cell lines by the International Stem Cell Initiative},
  author={Oluseun Adewumi and Behrouz Aflatoonian and Lars {\AA}hrlund-Richter and Michal Amit and Peter W. Andrews and Gemma Beighton and Paul Bello and Nissim Benvenisty and Lorraine S. Berry and Simon Bevan and Barak Blum and Justin Brooking and Kevin G. Chen and Andre Boon-Hwa Choo and Gary A. Churchill and Marie Corbel and Ivan Damjanov and J. S. Draper and Petr Dvoř{\'a}k and Katarina Emanuelsson and Roland A. Fleck and Angela Ford and Karin Gertow and Marina Gertsenstein and Paul J. Gokhale and Rebecca S. Hamilton and Ale{\vs} Hampl and Lyn E. Healy and Outi Hovatta and Johan Hyllner and Marta P. Imreh and Joseph Itskovitz‐Eldor and J. Jackson and Jacqueline L Johnson and Mark Jones and Kehkooi Kee and Benjamin L. King and Barbara B. Knowles and Majlinda Lako and Franck Lebrin and Barbara S. Mallon and Daisy Manning and Yoav Mayshar and Ronald D. G. McKay and Anna E. Michalska and Milla Mikkola and Masha Mileikovsky and Stephen L. Minger and Harry D.M. Moore and Christine L. Mummery and Andras Nagy and Norio Nakatsuji and Carmel O'brien and Steve Oh and Cia Olsson and Timo Otonkoski and Kye-yoon Park and Robert Passier and Hema Patel and Minal J. Patel and Roger A. Pedersen and Martin F. Pera and Marian S. Piekarczyk and Renee Reijo Pera and Benjamin E Reubinoff and Allan J. Robins and Janet Rossant and Peter J. Rugg-Gunn and Thomas C. Schulz and Henrik Semb and Eric S. Sherrer and Henrike Siemen and Glyn Stacey and Miodrag Stojkovic and Hirofumi Suemori and Jin P. Szatkiewicz and Tikva Turetsky and Timo Tuuri and S van den Brink and Kristina Vintersten and Sanna Vuoristo and Dorien Ward and Thomas Allan Weaver and Lesley Young and Weidong Zhang},
  journal={Nature Biotechnology},
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue… 

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Expression Patterns of Cancer-Testis Antigens in Human Embryonic Stem Cells and Their Cell Derivatives Indicate Lineage Tracks

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Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures.

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Differences between human and mouse embryonic stem cells.

Unique gene expression signatures of independently-derived human embryonic stem cell lines.

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Gene expression in human embryonic stem cell lines: unique molecular signature.

The first detailed characterization of undifferentiated huES cells is presented and several novel ES cell-specific expressed sequence tags were identified and mapped to the human genome.

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The overlapping similarities and differences in gene expression profiles of human and mouse ES cells provide a foundation for a detailed and concerted dissection of the molecular and cellular mechanisms governing their pluripotency, directed differentiation into specific cell types, and extended ability for self‐renewal.

Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001.

Recovered and expanded the earliest available cryopreserved stocks of the BG01, BG02, and BG03 hESC lines, which exhibited multiple definitive characteristics of undifferentiated cells, including long-term self-renewal, expression of markers of pluripotency, and maintenance of a normal karyotype.

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The issues of studying existing hESC lines made after August 9, 2001, deriving additional hESS lines, SCNT, and the possibility of generating clones or chimeras of human individuals have raised additional “hot” moral and ethical issues.

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The present study provides a molecular basis for the identity of human ES cells and demonstrates that during their in vitro differentiation they express embryonic specific genes in a stage specific manner.

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A stringent direct comparison between data sets obtained from two human ESCs are reported in order to obtain a list of genes that are enriched in ESCs and this work found 133 genes overlapped between the three pluripotent populations.