Characterization of human deoxycytidine kinase correlation with cDNA sequences

@article{Eriksson1991CharacterizationOH,
  title={Characterization of human deoxycytidine kinase correlation with cDNA sequences},
  author={Staffan K Eriksson and Ella Cederlund and Thomas Bergman and Hans J{\"o}rnvall and Christina Bohman},
  journal={FEBS Letters},
  year={1991},
  volume={280}
}
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Cloning of the Dck gene encoding rat deoxycytidine kinase.
Mammalian deoxyribonucleoside kinases.
Mimicking phosphorylation of Ser‐74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues
Binding of substrates to human deoxycytidine kinase studied with ligand-dependent quenching of enzyme intrinsic fluorescence.
TLDR
Fluorescence quenching was used to directly determine enzyme-ligand binding and revealed bimodal binding of dCTP, dCyd, dTTP, and dAdo and unimodalbinding of dUrd, and of d ado in the presence of 0.1 microM dCymru.
Activation of deoxycytidine kinase in lymphocytes is calcium dependent and involves a conformational change detectable by native immunostaining.
High incidence of alternatively spliced forms of deoxycytidine kinase in patients with resistant acute myeloid leukemia.
TLDR
Findings suggest that the presence of inactive, alternatively spliced dCK mRNA transcripts in resistant AML blasts may contribute to the process of AraC resistance in patients with AML.
Case study on the evolution of hetero-oligomer interfaces based on the differences in paralogous proteins
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The evolutionary trace of hetero-oligomer interfaces was addressed by comparing the structures of paralogous proteins by obtaining putative interfaces of the subunits in CPSF (cleavage and polyadenylation specificity factor), one of the human pre-mRNA 3'-processing complexes.
Engineering novel suicide enzymes for improved cancer gene therapy
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Two suicide gene therapy systems for enhanced prodrug activity are optimized and mutant fusion constructs carrying HSVTK mutants and MGMK are created, displaying superior tumor killing in vitro and impressive tumor growth inhibition in an animal tumor model at very low doses of GCV.
Protein Inhibitor of Activated STAT1 Interacts with and Up-regulates Activities of the Pro-proliferative Transcription Factor Krüppel-like Factor 5*
TLDR
Results indicate that PIAS1 is a functional partner of KLF5 and enhances the ability of KLf5 to promote proliferation in transfected cells.
Mitochondrial toxicity of nrti antiviral drugs: an integrated cellular perspective
TLDR
This review characterizes the pharmacological mechanisms and pathways that are involved in mitochondrial dysfunction caused by NRTIs, and suggests opportunities for future pharmacological research.
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References

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TLDR
The purification of deoxycytidine kinase from human leucemic spleen reported here result in a pure protein of molecular weight 28K, in agreement with earlier studies with partially purified calf thymus deoxyCytidine, but clearly different from some studies on human deoxy Cytidine Kinase.
Affinity purification of human deoxycytidine kinase: avoidance of structural and kinetic artifacts arising from limited proteolysis.
Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme
TLDR
The pure enzyme phosphorylates deoxycytidine, deoxyadenosine, and deoxyguanosine, demonstrating for the first time that the same enzyme molecule has the capacity to use these three nucleosides as substrates.
Human T lymphocytes express a protein-tyrosine kinase homologous to p56LSTRA.
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The study indicates that deoxycytidine kinase is a dimer with two subunits and has phosphorylating activity for deoxyguanosine, deoxyadenosine, cytidine, and cytosine arabinoside and this highly purified enzyme will facilitate the study of its regulation and phosphorylation of anticancer or antiviral nucleoside analogues.
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TLDR
Phylogenetic mapping of the conserved protein kinase catalytic domains can serve as a useful first step in the functional characterization of these newly identified family members.
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TLDR
It is proposed that this end product functions as a multisubstrate analogue, with its triphosphate groupbinding to the phosphate donor site of the enzyme and its deoxycytidine moiety overlapping and binding to the deoxynucleoside site in a highly specific manner.
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Drawings are made that the three classes of alcohol dehydrogenase reflect stages in the development of separate enzymes with distinct functional roles, and the similarity in the number of exchanges relative to that of the enzymes of the other two classes supports this conclusion.
Amino Acid Analysis by High Performance Liquid Chromatography of Phenylthiocarbamyl Derivatives
TLDR
Pre-column derivatization with phenylisothiocyanate to produce the phenylthiocarbamyl (PTC)-amino acids for subsequent separation by reverse phase HPLC has proved highly efficient and valuable.
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