Characterization of homocysteine γ-lyase from submerged and solid cultures of Aspergillus fumigatus ASH (JX006238).

@article{ElSayed2013CharacterizationOH,
  title={Characterization of homocysteine $\gamma$-lyase from submerged and solid cultures of Aspergillus fumigatus ASH (JX006238).},
  author={Ashraf S. A. El-Sayed and Salwa A. Khalaf and H. G. Abdel Aziz},
  journal={Journal of microbiology and biotechnology},
  year={2013},
  volume={23 4},
  pages={
          499-510
        }
}
Among 25 isolates, Aspergillus fumigatus ASH (JX006238) was identified as a potent producer of homocysteine gamma- lyase. The nutritional requirements to maximize the enzyme yield were optimized under submerged (SF) and solid-state fermentation (SSF) conditions, resulting in a 5.2- and 2.3-fold increase, respectively, after the last purification step. The enzyme exhibited a single homogenous band of 50 kDa on SDS-PAGE, along with an optimum pH of 7.8 and pH stability range of 6.5 to 7.8. It… 

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