Characterization of highly variable (GA/CT)n microsatellites in the bur oak, Quercus macrocarpa

@article{Dow2004CharacterizationOH,
  title={Characterization of highly variable (GA/CT)n microsatellites in the bur oak, Quercus macrocarpa},
  author={Beverly D. Dow and Mary V. Ashley and Henry F. Howe},
  journal={Theoretical and Applied Genetics},
  year={2004},
  volume={91},
  pages={137-141}
}
The objective of this study was to ascertain the usefulness of polymerase chain reaction (PCR)-based microsatellite analysis for studying pollination and parentage in a wind-pollinated temperate tree. A small insert genomic library of the bur oak (Quercus macrocarpa) was constructed and screened for the presence of (CA/GT)n and (GA/CT)n repeats. The proportion of positive clones yielded estimates of 3×105 such dinucleotide repeats per genome, roughly comparable to abundances reported in other… 
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