Characterization of high affinity and stereospecific [ (3)H ]d- fenfluramine binding to rat brain.

@article{Mennini1988CharacterizationOH,
  title={Characterization of high affinity and stereospecific [ (3)H ]d- fenfluramine binding to rat brain.},
  author={Tiziana Mennini and Marco Gobbi and Carlo Taddei and Silvio Garattini},
  journal={Neurochemistry international},
  year={1988},
  volume={13 3},
  pages={345-51}
}
Specific binding of [(3)H]d- fenfluramine , an anorectic agent acting on serotonergic system, was characterized in rat total brain minus cerebellum. The binding was reversible, stereospecific and with high affinity (K(d) about 80 nM). The receptor density in total brain was about 32 pmol/g tissue. Subcellular distribution showed a preferential localization in the microsomal and membrane fractions. The pharmacological characterization of [(3)H]d- fenfluramine specific binding showed that the d… CONTINUE READING