Characterization of hexamerin proteins and their mRNAs in the adult lubber grasshopper: The effects of nutrition and juvenile hormone on their levels.

@article{Hathaway2009CharacterizationOH,
  title={Characterization of hexamerin proteins and their mRNAs in the adult lubber grasshopper: The effects of nutrition and juvenile hormone on their levels.},
  author={Marianne Hathaway and John D. Hatle and Sen Li and X Ding and Thomas Barry and Fang Hong and Heidi Wood and D T Borst},
  journal={Comparative biochemistry and physiology. Part A, Molecular & integrative physiology},
  year={2009},
  volume={154 3},
  pages={323-32}
}
The lubber grasshopper (Romalea microptera) has three major hemolymph proteins with apparent sizes on native PAGE of 90, 270, and 500kDa and subunit sizes (79, 81 and 82kDa respectively) determined by SDS-PAGE . Trypsin fragments from each protein band were sequenced, used to design degenerate primers to amplify core cDNA fragments, which were extended by 5' and 3' RACE. All three cDNAs were closely related to insect hexamerins, had an N-terminal signal sequence, and their transcripts were… CONTINUE READING

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