Characterization of heterotrophic prokaryote subgroups in the Sfax coastal solar salterns by combining flow cytometry cell sorting and phylogenetic analysis

@inproceedings{Trigui2011CharacterizationOH,
  title={Characterization of heterotrophic prokaryote subgroups in the Sfax coastal solar salterns by combining flow cytometry cell sorting and phylogenetic analysis},
  author={Hana Trigui and Salma Masmoudi and C{\'e}line Brochier-Armanet and Aude Barani and G{\'e}rald Gr{\'e}gori and Michel Denis and Sam Dukan and Sami Maalej},
  booktitle={Extremophiles},
  year={2011}
}
Here, we combined flow cytometry (FCM) and phylogenetic analyses after cell sorting to characterize the dominant groups of the prokaryotic assemblages inhabiting two ponds of increasing salinity: a crystallizer pond (TS) with a salinity of 390 g/L, and the non-crystallizer pond (M1) with a salinity of 200 g/L retrieved from the solar saltern of Sfax in Tunisia. As expected, FCM analysis enabled the resolution of high nucleic acid content (HNA) and low nucleic acid content (LNA) prokaryotes… CONTINUE READING

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