Characterization of heat-shock proteins in Escherichia coli strains under thermal stress in vitro.

@article{UrbanChmiel2013CharacterizationOH,
  title={Characterization of heat-shock proteins in Escherichia coli strains under thermal stress in vitro.},
  author={Renata Urban-Chmiel and Marta Dec and Andrzej Puchalski and Andrzej Wernicki},
  journal={Journal of medical microbiology},
  year={2013},
  volume={62 Pt 12},
  pages={
          1897-901
        }
}
The aim of this study was to evaluate the effect of heat stress in in vitro conditions on the induction of heat-shock protein (Hsp)70 by Escherichia coli cells, and to determine the localization of Hsps in cell fractions. The material consisted of wild strains of E. coli isolated from the digestive tract of calves, suspended in an exponential-phase culture and subjected to 41.5 °C for 2 h. Individual fractions were analysed by SDS-PAGE and two-dimensional electrophoresis. Western blotting with… 

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