Characterization of glycosyl hydrolase family 3 beta-N-acetylglucosaminidases from Thermotoga maritima and Thermotoga neapolitana.

@article{Choi2009CharacterizationOG,
  title={Characterization of glycosyl hydrolase family 3 beta-N-acetylglucosaminidases from Thermotoga maritima and Thermotoga neapolitana.},
  author={Kyoung Hwa Choi and Ja Yeong Seo and Kyung-min Park and Cheon-Seok Park and Jaeho Cha},
  journal={Journal of bioscience and bioengineering},
  year={2009},
  volume={108 6},
  pages={
          455-9
        }
}
  • K. Choi, J. Seo, +2 authors J. Cha
  • Published 2009
  • Biology, Medicine
  • Journal of bioscience and bioengineering
The genes encoding beta-N-acetylglucosaminidase (nagA and cbsA) from Thermotoga maritima and Thermotoga neapolitana were cloned and expressed in Escherichia coli in order to investigate whether Thermotoga sp. is capable of utilizing chitin as a carbon source. NagA and CbsA were purified to homogeneity by HiTrap Q HP and Sephacryl S-200 HR column chromatography. Both enzymes were homodimers containing a family 3 glycoside hydrolase (GH3) catalytic domain, with a monomer molecular mass of 54 kDa… Expand
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