Characterization of glucoamylase from Lactobacillus amylovorus ATCC 33621

  title={Characterization of glucoamylase from Lactobacillus amylovorus ATCC 33621},
  author={Jennylynd A. James and Byong Hoon Lee},
  journal={Biotechnology Letters},
An intracellular glucoamylase, purified from Lactobacillus amylovorus, reacted selectively with polysaccharides. Kinetic studies indicated low affinity for maltose and maltotriose (Km 58 g/ml and 178 g/ml) and higher affinity for starch and dextrin (Km 0.01 g/ml and 0.02 g/ml). Glucoamylase was inhibited almost 50% by 10 mM glucose. Cu2+ and Pb2+ inhibited glucoamylase at 1.0 mM but EDTA and other metal chelators had no effect on the enzyme activity. Acarbose and Tris inhibited the enzyme by 84… CONTINUE READING


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