Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing

@inproceedings{Vandewalle2015CharacterizationOG,
  title={Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing},
  author={Kristof Vandewalle and Nele Festjens and Evelyn Plets and Marnik Vuylsteke and Yvan Saeys and Nico 3 Callewaert},
  booktitle={Nature communications},
  year={2015}
}
Reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consuming. Here, we describe a robust, easy to implement parallel sequencing-based method (Cartesian Pooling-Coordinate Sequencing or CP-CSeq) that reports both on the identity as well as on the location… CONTINUE READING
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The novel sequencing data generated in this study has been deposited at NCBI’s Sequence Read Archive under project code SRP056542

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Supplementary Information accompanies this paper at http://www.nature.com/ naturecommunications Competing financial interests: The authors declare no competing financial interests. Reprints and permission information is available online at http://npg.nature.com/ reprintsandpermissions/ How to cite t • 2015

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