Characterization of genetic diversity of Bacillus anthracis in France by using high-resolution melting assays and multilocus variable-number tandem-repeat analysis.

@article{Derzelle2011CharacterizationOG,
  title={Characterization of genetic diversity of Bacillus anthracis in France by using high-resolution melting assays and multilocus variable-number tandem-repeat analysis.},
  author={Sylviane Derzelle and S{\'e}verine Laroche and Philippe Le Fl{\`e}che and Yolande Hauck and Simon E. B. Thierry and Gilles Vergnaud and Nora Madani},
  journal={Journal of clinical microbiology},
  year={2011},
  volume={49 12},
  pages={4286-92}
}
Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype a set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome of Bacillus anthracis. SNP discrimination assays were performed in monoplex or duplex and applied to 100 B. anthracis isolates collected in France from 1953 to 2009 and a few reference strains. HRM provided a reliable and cheap alternative to subtype B. anthracis into one of the 12 major sublineages or… CONTINUE READING
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