Characterization of gating and peptide block of mSlo, a cloned calcium-dependent potassium channel.

@article{Sullivan1997CharacterizationOG,
  title={Characterization of gating and peptide block of mSlo, a cloned calcium-dependent potassium channel.},
  author={Donald E. Segal Edward John Allera Theodore M. Sullivan and Mats H. Holmqvist and Irwin B. Levitan},
  journal={Journal of neurophysiology},
  year={1997},
  volume={78 6},
  pages={2937-50}
}
The 20 amino acid Shaker inactivation peptide blocks mSlo, a cloned calcium-dependent potassium channel. Changing the charge and degree of hydrophobicity of the peptide alters its blocking kinetics. A "triple mutant" mSlo channel was constructed in which three amino acids (T256, S259, and L262), equivalent to those identified as part of the peptide's receptor site in the S4-S5 cytoplasmic loop region of the Shaker channel, were mutated simultaneously to alanines. These mutations produce only… CONTINUE READING

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