Characterization of gangliosides of epithelial cells of calf small intestine, with special reference to receptor-active sequences for enteropathogenic Escherichia coli K99.

@article{Teneberg1994CharacterizationOG,
  title={Characterization of gangliosides of epithelial cells of calf small intestine, with special reference to receptor-active sequences for enteropathogenic Escherichia coli K99.},
  author={Susann Teneberg and Peter T J Willemsen and F K de Graaf and Gunnar Stenhagen and Weston Pimlott and P A Jovall and Jonas {\AA}ngstr{\"o}m and K. A. Karlsson},
  journal={Journal of biochemistry},
  year={1994},
  volume={116 3},
  pages={
          560-74
        }
}
Glycolipids were prepared from epithelial cells of the small intestine of a newborn calf and assayed for Escherichia coli K99 binding activity on thin-layer chromatograms and in microtiter wells. The bacteria did not bind to any of the non-acid glycolipids, while in the acid fraction several binding-positive glycolipids were detected. The acid glycolipids were isolated and characterized by mass spectrometry, proton NMR spectroscopy and other methods. The following gangliosides were identified… CONTINUE READING

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