Characterization of flow cytometer instrument sensitivity.

  title={Characterization of flow cytometer instrument sensitivity.},
  author={Robert A. Hoffman and James C S Wood},
  journal={Current protocols in cytometry},
  volume={Chapter 1},
Fluorescence sensitivity, measured in terms of resolution, allows the researcher to more accurately answer the question of how dim a cell can be and still be resolvable from another population. This measure focuses on the width, i.e., the standard deviation, of the population distributions and not on the location, i.e., the mean intensity, of populations on a histogram scale relative to the background. To determine the fluorescence sensitivity in terms of the resolution, both the detection… CONTINUE READING