Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS.

  title={Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS.},
  author={Dimitrios Tsimogiannis and Martina Samiotaki and George N Panayotou and Vassiliki Oreopoulou},
  volume={12 3},
HPLC-DAD coupled with mass spectrometry in the positive ionization mode was applied to study the fragmentation of twelve selected flavonoids. Compounds belonging to all the major subgroups found in common plants, i.e. flavonols, flavones, dihydroflavonols, flavanones and flavanols were studied. Compound standards were injected into the spectrometer and produced characteristic mass spectra. The fragmentation of each compound was studied and it was shown that the dehydration and carbon monoxide… CONTINUE READING