Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition

@article{Yokoyama2017CharacterizationOE,
  title={Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition},
  author={Fumiaki Yokoyama and Jun Kawamoto and Tomoya Imai and Tatsuo Kurihara},
  journal={Extremophiles},
  year={2017},
  volume={21},
  pages={723-731}
}
A cold-adapted bacterium, Shewanella livingstonensis Ac10, which produces eicosapentaenoic acid (EPA) as a component of its membrane phospholipids, is useful as a model to study the function of EPA and as a host for heterologous production of thermolabile proteins at low temperatures. In this study, we characterized extracellular membrane vesicles (EMVs) of this bacterium to examine the involvement of EPA in the biogenesis of EMVs and for the future application of EMVs to extracellular protein… 
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References

SHOWING 1-10 OF 29 REFERENCES
Eicosapentaenoic Acid Plays a Beneficial Role in Membrane Organization and Cell Division of a Cold-Adapted Bacterium, Shewanella livingstonensis Ac10
TLDR
The results suggest that EPA in S. livingstonensis Ac10 is not required for bulk bilayer fluidity but plays a beneficial role in membrane organization and cell division at low temperatures, possibly through specific interaction between EPA and proteins involved in these cellular processes.
New Type of Outer Membrane Vesicle Produced by the Gram-Negative Bacterium Shewanella vesiculosa M7T: Implications for DNA Content
TLDR
The data demonstrate the existence of a previously unobserved type of double-bilayer OMV in the Gram-negative bacterium Shewanella vesiculosa M7T that can incorporate DNA, for which the name outer-inner membrane vesicle (O-IMV) is proposed.
Membrane Vesicles: A Common Feature in the Extracellular Matter of Cold-Adapted Antarctic Bacteria
TLDR
Differences were observed in the expression of some proteins depending on incubation temperature but further studies will be necessary to define their roles and implications in the survival of bacteria in the extreme Antarctic environment.
Deletion of degQ gene enhances outer membrane vesicle production of Shewanella oneidensis cells
TLDR
Analysis of periplasmic proteins from the resulting S. oneidensis strain showed that deletion of degQ induced protein accumulation and resulted in a significant decrease in protease activity within the periplasmsic space, suggesting that deletion may be responsible for the increase in envelope stress.
Identification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis
TLDR
A method for separating the inner and outer membranes by sucrose density gradient ultracentrifugation is established and performed proteomic studies of the inner membrane fraction and identified cold-inducible proteins that are presumed to be involved in chemotaxis, membrane protein biogenesis, and morphogenesis.
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the
Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium, Shewanella livingstonensis Ac10
TLDR
The Shewanella livingstonensis Ac10 strain has been used as a model organism for the investigation of microbial cold-adaptation mechanisms, and the EPA-less mutant of this strain exhibits cold-sensitive phenotypes, which include growth retardation, defect in cell.
Outer Membrane Vesicle Production by Escherichia coli Is Independent of Membrane Instability
TLDR
Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesICulation levels, demonstrating that vesicle production is not predictive of envelope instability.
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