Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition

  title={Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid composition},
  author={Fumiaki Yokoyama and Jun Kawamoto and Tomoya Imai and Tatsuo Kurihara},
A cold-adapted bacterium, Shewanella livingstonensis Ac10, which produces eicosapentaenoic acid (EPA) as a component of its membrane phospholipids, is useful as a model to study the function of EPA and as a host for heterologous production of thermolabile proteins at low temperatures. In this study, we characterized extracellular membrane vesicles (EMVs) of this bacterium to examine the involvement of EPA in the biogenesis of EMVs and for the future application of EMVs to extracellular protein… 
Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM13
This study showed that the induction of vesicle production and suppression of biofilm formation in response to lysine concentration are under the control of the same putative sensor protein.
Isolation of a Novel Bacterial Strain Capable of Producing Abundant Extracellular Membrane Vesicles Carrying a Single Major Cargo Protein and Analysis of Its Transport Mechanism
This study isolated a cold-adapted bacterium, Shewanella vesiculosa HM13, which abundantly produces EMVs, characterized them, and analyzed their cargo transport mechanism, indicating that the EMVs are produced even in the absence of the major cargo protein.
A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation
It is shown that overexpression of YihG in E. coli carrying a temperature-sensitive mutation in plsC allowed its growth at non-permissive temperatures, and analysis of the fatty acyl composition of PLs from the yihG-deletion mutant (∆yihG) revealed that endogenousYihG introduces the cis-vaccenoyl group into the sn-2 position of PLS.
Eco-evolutionary feedbacks mediated by bacterial membrane vesicles
This review presents current knowledge about BMVs in the context of bacterial eco-evolutionary dynamics among different environments and hosts to extend and deliver phenotypic traits that can have ecological and evolutionary value to both their producers and the ecosystem as a whole.
Environmental and taxonomic drivers of bacterial extracellular vesicle production in marine ecosystems
Using quantitative analysis of marine microbial cultures, the data indicate that different marine taxa release vesicles at rates varying across an order of magnitude, and that vesicle production can change dynamically as a function of environmental conditions.
Cracking Open Bacterial Membrane Vesicles
This review will discuss recent progress in understanding the biochemical composition and properties of MVs, and show the potential for applications in various fields.
Outer Membrane Vesicles of Gram-Negative Bacteria: An Outlook on Biogenesis
This review covers the most recent information on cellular components involved in OMV biogenesis, such as lipoproteins and outer membrane proteins, lipopolysaccharide, phospholipids, quorum-sensing molecules, and flagella.


Eicosapentaenoic Acid Plays a Beneficial Role in Membrane Organization and Cell Division of a Cold-Adapted Bacterium, Shewanella livingstonensis Ac10
The results suggest that EPA in S. livingstonensis Ac10 is not required for bulk bilayer fluidity but plays a beneficial role in membrane organization and cell division at low temperatures, possibly through specific interaction between EPA and proteins involved in these cellular processes.
New Type of Outer Membrane Vesicle Produced by the Gram-Negative Bacterium Shewanella vesiculosa M7T: Implications for DNA Content
The data demonstrate the existence of a previously unobserved type of double-bilayer OMV in the Gram-negative bacterium Shewanella vesiculosa M7T that can incorporate DNA, for which the name outer-inner membrane vesicle (O-IMV) is proposed.
Membrane Vesicles: A Common Feature in the Extracellular Matter of Cold-Adapted Antarctic Bacteria
Differences were observed in the expression of some proteins depending on incubation temperature but further studies will be necessary to define their roles and implications in the survival of bacteria in the extreme Antarctic environment.
Deletion of degQ gene enhances outer membrane vesicle production of Shewanella oneidensis cells
Analysis of periplasmic proteins from the resulting S. oneidensis strain showed that deletion of degQ induced protein accumulation and resulted in a significant decrease in protease activity within the periplasmsic space, suggesting that deletion may be responsible for the increase in envelope stress.
Identification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis
A method for separating the inner and outer membranes by sucrose density gradient ultracentrifugation is established and performed proteomic studies of the inner membrane fraction and identified cold-inducible proteins that are presumed to be involved in chemotaxis, membrane protein biogenesis, and morphogenesis.
Outer-Inner Membrane Vesicles Naturally Secreted by Gram-Negative Pathogenic Bacteria
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the
Characterization of 1-acyl-sn-glycerol-3-phosphate acyltransferase from a polyunsaturated fatty acid-producing bacterium, Shewanella livingstonensis Ac10
The Shewanella livingstonensis Ac10 strain has been used as a model organism for the investigation of microbial cold-adaptation mechanisms, and the EPA-less mutant of this strain exhibits cold-sensitive phenotypes, which include growth retardation, defect in cell.
Outer Membrane Vesicle Production by Escherichia coli Is Independent of Membrane Instability
Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesICulation levels, demonstrating that vesicle production is not predictive of envelope instability.