Characterization of extra- and intracellular phytases from Rhizopus oligosporus used in tempeh production.

@article{Sutardi1988CharacterizationOE,
  title={Characterization of extra- and intracellular phytases from Rhizopus oligosporus used in tempeh production.},
  author={Sutardi and Kenneth A Buckle},
  journal={International journal of food microbiology},
  year={1988},
  volume={6 1},
  pages={67-79}
}
Rhizopus oligosporus strain CT11K2, commonly used in tempeh (fermented soybean) production produced both extra- and intracellular phytases. The enzymes were isolated from growth media and the cultured mould and partially purified by acetone fractionation, gel filtration on Sephadex G-100 and DEAE-cellulose chromatography. Intracellular phytase activity was higher than that of the extracellular enzyme. Both enzymes showed maximum activity at pH 4.5 and 55 degrees C, suggesting relatively high… CONTINUE READING

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