Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes.

@article{Bouchard1990CharacterizationOE,
  title={Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes.},
  author={Robert A. Bouchard},
  journal={Genome},
  year={1990},
  volume={33 1},
  pages={
          68-79
        }
}
The inserts of plasmid cDNA clones for transcripts showing meiotic prophase specific expression show cross reassociation to varying degrees of intensity with one another. These clones were recovered from a cDNA library made from Lilium microsporocyte poly(A)+ RNA. RNA-dot and Northern-blot analyses indicate that these clones represent transcripts specific to the meiotic prophase interval in microsporocytes. The transcripts appear to constitute the most abundant class of meiosis-specific poly(A… 

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
Developmental regulation of specific, cytoplasmic, plant LMW HSPs are demonstrated, suggesting also their involvement in water-stress tolerance.

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TLDR
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...

References

SHOWING 1-10 OF 28 REFERENCES

Changes in poly(a)+ RNA during male meiosis in Lilium.

TLDR
Both methods indicate that there is a very low of poly(A)+ RNA synthesis throughout prophase, and these events are considered in terms of the juncture at which they occur in the plant life-cycle.

Cloning and analysis of cDNA sequences coding for two 16 kilodalton heat shock proteins (hsps) in Caenorhabditis elegans: homology with the small hsps of Drosophila.

TLDR
Two-dimensional gel analysis of in vitro synthesized hsp16 reveals the existence of five distinct components of identical molecular weights, but with different isoelectric points.

Nucleic acid metabolism in the nucleus and cytoplasm of angiosperm meiocytes.

TLDR
Evidence is presented indicating that a large proportion of the m- and rRNA is purged from the meiocyte cytoplasm by a combination of normal degradation and the activity of specific hydrolases, suggesting that there is at least one stage in the life cycle of all plants at which a purge of information-carrying molecules takes place.

The ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene react very differently to perturbations of DNA sequence

The effect of various types of DNA sequence alterations on the activity of the ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene was studied by P

Composition and role of the synaptonemal complex.

TLDR
The role of the synaptonemal complex (SC) in synapsis during meiotic prophase is examined in spermatocytes and oocytes of mice heterozygous for rearrangements, using light and electron microscopy of whole mount spreads to provide a morphological time axis for synaptic events.

Characterization of the prosome from Drosophila and its similarity to the cytoplasmic structures formed by the low molecular weight heat‐shock proteins.

TLDR
A ribonucleoprotein structure is identified from the cytoplasm of Drosophila melanogaster tissue culture cells which is equivalent to the prosome, a recently described ribon nucleoprotein particle of duck and mouse cells, and the partial nucleotide sequences of several small RNA species associated with the Dosophila prosome.

DNA sequence and transcript mapping of a soybean gene encoding a small heat shock protein.

TLDR
Analysis of hydropathy profiles suggests that the four small heat shock proteins of Drosophila show conservation of several major hydrophilic and hydrophobic regions, which suggests that these proteins have common structural features.

Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family

TLDR
Analysis of the DNA sequences in the 5' flanking regions of the soybean genes with the corresponding regions of Drosophila melanogaster HS-protein genes revealed striking similarity between plants and animals in the presumptive promoter structure of thermoinducible genes.

Genetic control of meiosis.