Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes.

  title={Characterization of expressed meiotic prophase repeat transcript clones of Lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes.},
  author={Robert A. Bouchard},
  volume={33 1},
The inserts of plasmid cDNA clones for transcripts showing meiotic prophase specific expression show cross reassociation to varying degrees of intensity with one another. These clones were recovered from a cDNA library made from Lilium microsporocyte poly(A)+ RNA. RNA-dot and Northern-blot analyses indicate that these clones represent transcripts specific to the meiotic prophase interval in microsporocytes. The transcripts appear to constitute the most abundant class of meiosis-specific poly(A… 

Characterization of cDNAs induced in meiotic prophase in lily microsporocytes.

To identify and analyze genes functioning during reproductive cell formation in higher plants, cDNAs harboring the messages induced in meiotic prophase were isolated and characterized and showed similarity with known gene products, e.g. heat shock proteins, serine proteases in Bacillus and RAD 51 gene product in yeast.

Isolation and characterization of a small heat shock protein gene from maize.

It is concluded that this maize genomic DNA clone, recovered through its homology to clones for meiotic transcripts in lily, represents a genuine maize small heat shock protein gene.

An early meiosis cDNA clone from wheat

A clone (designated pAWJL3) has been isolated from a cDNA library generated from RNA prepared from young wheat florets at early meiosis, which represents a small family of genes with about 20 members located on the short arms of group 3 and 5 chromosomes.

The independent stage-specific expression of the 18-kDa heat shock protein genes during microsporogenesis in Zea mays L.

These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response.

Characterization of a Meiosis-Associated Heat Shock Protein 70

The results suggest that LIM18 is a meiosis associated HSP70 Iike protein that may be involved in nuclear and/or chromosome events during meiosis Iof microsporogenesis.

Sequence, identification and characterization of cDNAs encoding two different members of the 18 kDa heat shock family of Zea mays L.

It is substantiated that at least two of the 18 kDa hsps in maize are products of different but related genes and that transcripts for these proteins accumulate during heat shock and that both their nucleotide and deduced amino acid sequences share extensive similarities with the class VI small hsPS in soybean and with transcripts expressed during meiosis in Lilium.

Cloning and molecular characterization of a strawberry fruit ripening-related cDNA corresponding a mRNA for a low-molecular-weight heat-shock protein

The results clearly show a specific relationship of this njjs4 strawberry gene with the processes of seed maturation and fruit ripening, and strongly support that at least some of the class-I LMW heat-shock protein-like genes have a heat-stress-independent role in plant development, including fruit ripens.

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

Developmental regulation of specific, cytoplasmic, plant LMW HSPs are demonstrated, suggesting also their involvement in water-stress tolerance.

Translation of some maize small heat shock proteins is initiated from internal in-frame AUGs.

The fact that these genes do not contain introns, coupled with the observations reported herein, suggest that maize may have established another method of using a single gene to produce a number of different proteins.



Changes in poly(a)+ RNA during male meiosis in Lilium.

Both methods indicate that there is a very low of poly(A)+ RNA synthesis throughout prophase, and these events are considered in terms of the juncture at which they occur in the plant life-cycle.

Cloning and analysis of cDNA sequences coding for two 16 kilodalton heat shock proteins (hsps) in Caenorhabditis elegans: homology with the small hsps of Drosophila.

Two-dimensional gel analysis of in vitro synthesized hsp16 reveals the existence of five distinct components of identical molecular weights, but with different isoelectric points.

Nucleic acid metabolism in the nucleus and cytoplasm of angiosperm meiocytes.

Evidence is presented indicating that a large proportion of the m- and rRNA is purged from the meiocyte cytoplasm by a combination of normal degradation and the activity of specific hydrolases, suggesting that there is at least one stage in the life cycle of all plants at which a purge of information-carrying molecules takes place.

The ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene react very differently to perturbations of DNA sequence

The effect of various types of DNA sequence alterations on the activity of the ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene was studied by P

Composition and role of the synaptonemal complex.

The role of the synaptonemal complex (SC) in synapsis during meiotic prophase is examined in spermatocytes and oocytes of mice heterozygous for rearrangements, using light and electron microscopy of whole mount spreads to provide a morphological time axis for synaptic events.

Characterization of the prosome from Drosophila and its similarity to the cytoplasmic structures formed by the low molecular weight heat‐shock proteins.

A ribonucleoprotein structure is identified from the cytoplasm of Drosophila melanogaster tissue culture cells which is equivalent to the prosome, a recently described ribon nucleoprotein particle of duck and mouse cells, and the partial nucleotide sequences of several small RNA species associated with the Dosophila prosome.

DNA sequence and transcript mapping of a soybean gene encoding a small heat shock protein.

Analysis of hydropathy profiles suggests that the four small heat shock proteins of Drosophila show conservation of several major hydrophilic and hydrophobic regions, which suggests that these proteins have common structural features.

Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family

Analysis of the DNA sequences in the 5' flanking regions of the soybean genes with the corresponding regions of Drosophila melanogaster HS-protein genes revealed striking similarity between plants and animals in the presumptive promoter structure of thermoinducible genes.

Genetic control of meiosis.