Characterization of excretory-secretory products from larval stages of Haemonchus contortus cultured in vitro.

@article{Gamble1996CharacterizationOE,
  title={Characterization of excretory-secretory products from larval stages of Haemonchus contortus cultured in vitro.},
  author={Howard R Gamble and Linda S. Mansfield},
  journal={Veterinary parasitology},
  year={1996},
  volume={62 3-4},
  pages={
          291-305
        }
}
To determine biochemical changes associated with early parasite development, Haemonchus contortus larvae were cultured in vitro to the fourth stage (L4). Infective larvae developed from third to fourth stage in 48-96 h. Metabolic activity increased following stimulus of infective stages by CO2 secretion/excretion of significant amounts of protein into cultures and larval feeding did not occur until larvae had molted to the fourth stage. Larval feeding, as monitored by the ability of larvae to… CONTINUE READING
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