Characterization of epoxide hydrolase activity in shape Alternaria alternata f. sp. shape lycopersici. Possible involvement in toxin production

@article{Pinot2004CharacterizationOE,
  title={Characterization of epoxide hydrolase activity in shape Alternaria alternata f. sp. shape lycopersici. Possible involvement in toxin production},
  author={Franck Pinot and Elo{\'i}sa Dutra Caldas and Christina Schmidt and David G Gilchrist and A. Daniel Jones and Bruce D Hammock and Carl K. Winter},
  journal={Mycopathologia},
  year={2004},
  volume={140},
  pages={51-58}
}
Using trans-diphenylpropane oxide (tDPPO) as a substrate, we measured epoxide hydrolase (EH) activity in subcellular fractions of Alternaria alternata f. sp. lycopersici (Aal), a fungus that produces host-specific toxins. The activity was mainly (>99.5%) located in the soluble fraction (100,000 × g supernatant) with the optimum pH at 7.4. An increase of… CONTINUE READING