Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.

@article{BarnaVetr1979CharacterizationOE,
  title={Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.},
  author={I Barna-Vetr{\'o} and Raquel Varro and E Osz},
  journal={Annales immunologiae Hungaricae},
  year={1979},
  volume={19},
  pages={143-8}
}
In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not… CONTINUE READING