Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.

@article{Hryhorenko1998CharacterizationOE,
  title={Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.},
  author={E A Hryhorenko and K Rittenhouse-Diakun and Njata Harvey and Janet Morgan and Carleton C. Stewart and Allan R. Oseroff},
  journal={Photochemistry and photobiology},
  year={1998},
  volume={67 5},
  pages={565-72}
}
Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects of ALA and ALA-PDT on resting and activated human peripheral blood T lymphocytes. Accumulation of PpIX depends inversely on the rate of its iron-dependent conversion into heme… CONTINUE READING