• Corpus ID: 89645384

Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis

  title={Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis},
  author={Lesibana Anthony Malinga},
First report of whole-genome analysis of an extensively drug-resistant Mycobacterium tuberculosis clinical isolate with bedaquiline, linezolid and clofazimine resistance from Uganda
This is the first identification of extensively drug-resistant Mycobacterium tuberculosis clinical isolates with bedaquiline, linezolid and clofazimine resistance from Uganda, and strongly highlights the importance of combating DR-TB in Africa through implementing next generation sequencing that can test resistance to all drugs while providing a faster turnaround time.
In Silico Approach for Phytocompound-Based Drug Designing to Fight Efflux Pump-Mediated Multidrug-Resistant Mycobacterium tuberculosis
The mechanism and appearance of drug resistance in Mycobacterium tuberculosis with emphasis on efflux pumps along with the significance of phytochemicals as inhibitors of these pumps and their screening strategy by computational approaches are highlighted.
Molecular modelling and simulation studies of the Mycobacterium tuberculosis multidrug efflux pump protein Rv1258c
An accurate 3D model of Rv1258c is generated using MODELLER and its structure is validated using molecular dynamic simulation studies with GROMACS software and Spectinamide was found to bind to a different location on the outside surface of the protein suggesting its ability to avoid the efflux channel.


The diagnostic accuracy of the GenoType® Mtbdrsl assay for the detection of resistance to second-line anti-tuberculosis drugs
The influence of different reference standards was explored and the pooled sensitivity and specificity of MTBDRsl for FQ resistance, SLID resistance, and XDR-TB was estimated.
Evaluation of Genotype MTBDRsl Assay to Detect Drug Resistance Associated with Fluoroquinolones, Aminoglycosides and Ethambutol on Clinical Sediments
Genotype MTBDRsl is simple, rapid, economical assay that can be used to detect commonly known resistance associated with Fluoroquinolone, second line injectable drugs and ethambutol and detects the targeted resistance in less time as compared to phenotypic DST.
Rifampicin reduces susceptibility to ofloxacin in rifampicin-resistant Mycobacterium tuberculosis through efflux.
Exposure of rifampicin resistant M. tuberculosis strains to rifampsicin can potentially compromise the efficacy of the second-line treatment regimens containing ofloxacin, thereby emphasising the need for rapid diagnostics to guide treatment.
Evaluation of Genetic Mutations Associated with Mycobacterium tuberculosis Resistance to Amikacin, Kanamycin and Capreomycin: A Systematic Review
Background Rapid molecular diagnostics for detecting multidrug-resistant and extensively drug-resistant tuberculosis (M/XDR-TB) primarily identify mutations in Mycobacterium tuberculosis (Mtb) genes
The ATP synthase inhibitor bedaquiline interferes with small-molecule efflux in Mycobacterium smegmatis
The ATP synthase inhibitor bedaquiline interferes with small-molecule efflux in Mycobacterium smegmatis
clinical isolates of Mycobacterium tuberculosis
  • J Antimicrob Chemother
  • 2015
Whole-Genome Sequencing for Rapid Susceptibility Testing of M. tuberculosis
Widespread occurrence of Mycobacterium tuberculosis DNA from 18th–19th century Hungarians and the implications for tuberculosis treatment and control are studied.
Performance of the MTBDRsl assay in Georgia.
Although the MTBDRsl assay had a rapid turnaround time, detection of second-line drug resistance was poor compared to DST and further genetic mutations associated with resistance to second- line drugs should be included in the assay to improve test performance and clinical utility.