Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2.

@article{Soler1995CharacterizationOD,
  title={Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2.},
  author={Manuel Soler and A Plovins and Humberto Mart{\'i}n and Mar{\'i}a Molina and C{\'e}sar Nombela},
  journal={Molecular microbiology},
  year={1995},
  volume={17 5},
  pages={833-42}
}
MKK1/MKK2 and SLT2 (MPK1) are three Saccharomyces cerevisiae genes, coding for protein kinases, that have been postulated to act sequentially as part of the Pkc1p signalling pathway, a phosphorylation cascade essential for cell integrity. By using the 'two-hybrid system' and co-purification experiments on glutathione-agarose beads, we have shown that Slt2p interacts in vivo and in vitro with both Mkk1p and Mkk2p, thus confirming a previous suggestion based on epistasis experiments of the… CONTINUE READING

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