Characterization of deoxyuridine 5'-triphosphate nucleotidohydrolase from Trypanosoma cruzi.

@article{BernierVillamor2002CharacterizationOD,
  title={Characterization of deoxyuridine 5'-triphosphate nucleotidohydrolase from Trypanosoma cruzi.},
  author={Victor Bernier-Villamor and Ana L Camacho and Fernando Hidalgo-Zarco and J A S{\'a}nchez P{\'e}rez and Luis Miguel Ru{\'i}z-P{\'e}rez and Dolores Gonz{\'a}lez-Pacanowska},
  journal={FEBS letters},
  year={2002},
  volume={526 1-3},
  pages={147-50}
}
We report the cloning and kinetic characterization of Trypanosoma cruzi deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) whose coding sequence was isolated by genetic complementation in Escherichia coli. The deduced amino acid sequence was similar to Leishmania major dUTPase although it exhibits an amino acid insertion which is sensitive to protease inactivation. The catalytically active species of the enzyme is a dimer and a detailed kinetic characterization showed that it is highly… CONTINUE READING

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