Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.

@article{Demanche2009CharacterizationOD,
  title={Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.},
  author={Sandrine Deman{\`e}che and Laurent Philippot and Maude M. David and Elisabeth Navarro and Timothy M. Vogel and Pascal Simonet},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 2},
  pages={534-7}
}
We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters. 

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Ecology of denitrifying prokaryotes in agricultural soil

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