Characterization of dengue virus NS1 stably expressed in 293T cell lines.

  title={Characterization of dengue virus NS1 stably expressed in 293T cell lines.},
  author={Sansanee Noisakran and Thanyaporn Dechtawewat and Panthip Rinkaewkan and Chunya Puttikhunt and Amornrat Kanjanahaluethai and Watchara Kasinrerk and Nopporn Sittisombut and Prida Malasit},
  journal={Journal of virological methods},
  volume={142 1-2},
Dengue virus NS1 is a viral nonstructural protein detected in sera of infected individuals and in infected cells. Multiple NS1 structural forms have been reported but the functional characteristics of these forms remain unknown. In this study, a set of 293T cell lines stably expressing recombinant dengue NS1 without additional C-terminal sequence (rNS1s), with a heterologous transmembrane segment (rNS1tm), or with the 26-residue N-terminal portion of NS2A (rNS1v1) was established to aid in the… CONTINUE READING


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