Characterization of deletion and truncation mutants of the rat glucagon receptor. Seven transmembrane segments are necessary for receptor transport to the plasma membrane and glucagon binding.

@article{Unson1995CharacterizationOD,
  title={Characterization of deletion and truncation mutants of the rat glucagon receptor. Seven transmembrane segments are necessary for receptor transport to the plasma membrane and glucagon binding.},
  author={Cecilia G. Unson and Aaron M Cypess and Hee Nam Kim and Paul K. Goldsmith and C J Carruthers and R. B. Merrifield and Thomas P. Sakmar},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 46},
  pages={27720-7}
}
Glucagon receptor mutants were characterized with the aim of elucidating minimal structural requirements for proper biosynthesis, ligand binding, and adenylyl cyclase coupling. One N-terminal deletion mutant and five truncation mutants with progressively shorter C termini were expressed in transiently transfected monkey kidney (COS-1) cells. Each truncation mutant was designed so that the truncated C-terminal tail would remain on the cytoplasmic surface of the receptor. In order to characterize… CONTINUE READING