Characterization of cytokine expression induced by avian influenza virus infection with real-time RT-PCR.

@article{Kapczynski2014CharacterizationOC,
  title={Characterization of cytokine expression induced by avian influenza virus infection with real-time RT-PCR.},
  author={Darrell R. Kapczynski and Hai Jun Jiang and Michael H. Kogut},
  journal={Methods in molecular biology},
  year={2014},
  volume={1161},
  pages={
          217-33
        }
}
Knowledge of how birds react to infection from avian influenza virus is critical to understanding disease pathogenesis and host response. The use of real-time (R) RT-PCR to measure innate immunity, including cytokine and interferon gene expression, has become a standard technique employed by avian immunologists interested in examining these responses. This technique utilizes nucleotide primers and fluorescent reporter molecules to measure amplification of the gene of interest. The use of RRT… CONTINUE READING
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