Corpus ID: 31904732

Characterization of cytochrome P-450 2B6 in human liver microsomes.

@article{Mimura1993CharacterizationOC,
  title={Characterization of cytochrome P-450 2B6 in human liver microsomes.},
  author={M. Mimura and T. Baba and H. Yamazaki and S. Ohmori and Y. Inui and F. Gonzalez and F. Guengerich and T. Shimada},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1993},
  volume={21 6},
  pages={
          1048-56
        }
}
  • M. Mimura, T. Baba, +5 authors T. Shimada
  • Published 1993
  • Chemistry, Medicine
  • Drug metabolism and disposition: the biological fate of chemicals
A cytochrome P-450 (P-450) enzyme of the CYP2B subfamily was partially purified from human liver microsomes and characterized with respect to immunochemical properties, N-terminal amino acid sequence, and catalytic activities toward typical P-450 substrates. P-450 enzymes were monitored in chromatographic fractions by immunoblotting analysis using antibodies raised against a monkey P-450 2B, as well as several purified human P-450 enzymes. The final P-450 2B preparation thus obtained was… Expand
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