Characterization of cucurbita maxima phloem serpin-1 (CmPS-1). A developmentally regulated elastase inhibitor.

@article{Yoo2000CharacterizationOC,
  title={Characterization of cucurbita maxima phloem serpin-1 (CmPS-1). A developmentally regulated elastase inhibitor.},
  author={B C Yoo and Keiko Aoki and Yang Xiang and Leslie R Campbell and Richard J. Hull and Beatriz G. Xoconostle-Cazares and Jan Monzer and Joo Young Lee and Diane E. Ullman and William J. Lucas},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 45},
  pages={35122-8}
}
We report on the molecular, biochemical, and functional characterization of Cucurbita maxima phloem serpin-1 (CmPS-1), a novel 42-kDa serine proteinase inhibitor that is developmentally regulated and has anti-elastase properties. CmPS-1 was purified to near homogeneity from C. maxima (pumpkin) phloem exudate and, based on microsequence analysis, the cDNA encoding CmPS-1 was cloned. The association rate constant (k(a)) of phloem-purified and recombinant His(6)-tagged CmPS-1 for elastase was 3.5… CONTINUE READING

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