Characterization of corneal keratocyte morphology and mechanical activity within 3-D collagen matrices.

@article{Lakshman2010CharacterizationOC,
  title={Characterization of corneal keratocyte morphology and mechanical activity within 3-D collagen matrices.},
  author={Neema Lakshman and Areum Daseul Kim and Walter Matthew Petroll},
  journal={Experimental eye research},
  year={2010},
  volume={90 2},
  pages={350-9}
}
The purpose of this study was to assess quantitatively the differences in morphology, cytoskeletal organization and mechanical behavior between quiescent corneal keratocytes and activated fibroblasts in a 3-D culture model. Primary cultures of rabbit corneal keratocytes and fibroblasts were plated inside type I collagen matrices in serum-free media or 10% FBS, and allowed to spread for 1-5 days. Following F-actin labeling using phalloidin, and immunolabeling of tubulin, alpha-smooth muscle… CONTINUE READING