Characterization of conformers of D 1 of photosystem II using site-directed antibodies.

@article{Besford1990CharacterizationOC,
  title={Characterization of conformers of D 1 of photosystem II using site-directed antibodies.},
  author={Robert T. Besford and Barbara Massie Thomas and Neville S. Huskisson and Goeffrey W. Butcher},
  journal={Zeitschrift fur Naturforschung. C, Journal of biosciences},
  year={1990},
  volume={45 6},
  pages={621-6}
}
Antibodies have been raised to synthetic peptides, corresponding to a region in the loop spanning helices 4 and 5 of D 1 protein (Ala 250-Phe 265) and to a region anticipated to be near the C terminus of mature D 1 (His 332-Ala 345). Polyclonal antibodies to the sequence His 332-Ala 345 reacted with a 32 kDa polypeptide in thylakoid preparations identified as D 1 from its resistance (pea) or susceptibility (wheat) to lysine-C degradation. A monoclonal antibody to His 332-Ala 345 reacted… CONTINUE READING