Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

@article{Roos2012CharacterizationOC,
  title={Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.},
  author={Christian Joshua Roos and Michael Zocher and Daniel M{\"u}ller and Daniela M{\"u}nch and Tanja Schneider and H. -G. Sahl and Frank Scholz and Josef Wachtveitl and Yuliang Ma and Davide Proverbio and Erik Henrich and Volker D{\"o}tsch and Frank Doz. Dr.-Ing. Bernhard},
  journal={Biochimica et biophysica acta},
  year={2012},
  volume={1818 12},
  pages={
          3098-106
        }
}
Nanodiscs (NDs) enable the analysis of membrane proteins (MP) in natural lipid bilayer environments. In combination with cell-free (CF) expression, they could be used for the co-translational insertion of MPs into defined membranes. This new approach allows the characterization of MPs without detergent contact and it could help to identify effects of particular lipids on catalytic activities. Association of MPs with different ND types, quality of the resulting MP/ND complexes as well as… CONTINUE READING
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