Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.

@article{Liu2016CharacterizationOC,
  title={Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.},
  author={Jinyu Liu and Linting Huang and Yirong Wang and Ying Huang},
  journal={Microbiology},
  year={2016}
}
The endonuclease tRNase Z is responsible for the 3'-end processing of tRNA precursors, which is one of the essential steps in tRNA maturation. The fission yeast Schizosaccharomyces pombe contains two essential tRNase ZL genes (trz1 and trz2) involved in nuclear and mitochondrial tRNA 3'-end processing, respectively. Our previous studies suggest that trz2 is expressed at a very low level. Here we report characterization of the trz2 promoter. Using lacZ as a reporter, we show that the trz2… 
2 Citations
Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.
TLDR
The results suggest that trz2 overexpression may cause mitochondrial dysfunction, which is likely to lead to perturbation of iron homeostasis, ROS accumulation and induction of apoptotic cell death in S. pombe.
The fission yeast Schizosaccharomyces pombe Mtf2 is required for mitochondrial cox1 gene expression.
TLDR
The results suggest that unlike its counterpart in S. cerevisiae which plays a general role in synthesis of mtDNA-encoded proteins, S. pombe Mtf2 primarily functions in cox1 translation and the effect of mtf2 deletion on splicing of introns in mtDNA is likely due to a deficiency in the synthesis of intron-encoding maturases.

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