Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.

  title={Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.},
  author={Jinyu Liu and Linting Huang and Yirong Wang and Ying Huang},
The endonuclease tRNase Z is responsible for the 3'-end processing of tRNA precursors, which is one of the essential steps in tRNA maturation. The fission yeast Schizosaccharomyces pombe contains two essential tRNase ZL genes (trz1 and trz2) involved in nuclear and mitochondrial tRNA 3'-end processing, respectively. Our previous studies suggest that trz2 is expressed at a very low level. Here we report characterization of the trz2 promoter. Using lacZ as a reporter, we show that the trz2… 
2 Citations
Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.
The results suggest that trz2 overexpression may cause mitochondrial dysfunction, which is likely to lead to perturbation of iron homeostasis, ROS accumulation and induction of apoptotic cell death in S. pombe.
The fission yeast Schizosaccharomyces pombe Mtf2 is required for mitochondrial cox1 gene expression.
The results suggest that unlike its counterpart in S. cerevisiae which plays a general role in synthesis of mtDNA-encoded proteins, S. pombe Mtf2 primarily functions in cox1 translation and the effect of mtf2 deletion on splicing of introns in mtDNA is likely due to a deficiency in the synthesis of intron-encoding maturases.


The fission yeast Schizosaccharomyces pombe has two distinct tRNase Z(L)s encoded by two different genes and differentially targeted to the nucleus and mitochondria.
Evidence is provided for partitioning of tRNase Z function between two different proteins in S. pombe, although one cannot rule out specialized functions for each protein.
Partitioning of the Nuclear and Mitochondrial tRNA 3′-End Processing Activities between Two different Proteins in Schizosaccharomyces pombe *
The results provide a rare example showing partitioning of the nuclear and mitochondrial tRNase ZL activities between two different proteins in S. pombe, and advance the understanding of tRNA 3′-end maturation.
Rrn7 Protein, an RNA Polymerase I Transcription Factor, Is Required for RNA Polymerase II-dependent Transcription Directed by Core Promoters with a HomolD Box Sequence*
The results suggest that Rrn7 is an excellent candidate to be involved in the coordination of ribosomal DNA and ribosom gene transcription during ribosome synthesis and, therefore, offer a new perspective to study conservation and evolvability of regulatory networks in eukaryotes.
Functional conservation of tRNase ZL among Saccharomyces cerevisiae, Schizosaccharomyces pombe and humans.
The findings reveal that 3' end processing is a limiting step for defective tRNA maturation and demonstrate that overexpression of sptrz1(+), scTRZ1 and ELAC2 can promote defective tRNase Z 3' processing in vivo.
Identification and Sequence Analysis of Metazoan tRNA 3′-End Processing Enzymes tRNase Zs
The analysis supports the notion that in metazoans a single tRNase ZL has evolved to participate in both nuclear and mitochondrial tRNA 3′-end processing, whereas t RNase ZS may have evolved new functions.
The tRNase Z family of proteins: physiological functions, substrate specificity and structural properties
This review summarizes the current knowledge about tRNase Z enzymes, including detailed functional studies on purified recombinant enzymes, mutagenesis studies and the determination of the crystal structure of three bacterial enzymes.
U3 snoRNA promoter reflects the RNA’s function in ribosome biogenesis
Steady state measurements of cellular RNAs support the suggestion that the U3 snoRNA promoter may represent the fusion of two promoter systems reflecting the special role of this RNA in ribosome biogenesis.
Identification and analysis of candidate fungal tRNA 3'-end processing endonucleases tRNase Zs, homologs of the putative prostate cancer susceptibility protein ELAC2
The results support the proposal that t RNase ZL has evolved as a result of duplication and diversification of the tRNase ZS gene and shows the presence of pseudo-substrate recognition and catalytic motifs at the N-terminal halves of tRNA ZLs.
The CAGTCACA box in the fission yeast Schizosaccharomyces pombe functions like a TATA element and binds a novel factor.
Evidence is provided that this box, like a TATA sequence, is involved in basal expression and fixing the transcriptional start sites of these genes.
Conservation and divergence of transcriptional coregulations between box C/D snoRNA and ribosomal protein genes in Ascomycota.
The results indicate that coregulation via common cis elements is an important mechanism to coordinate expression of the RP and snoRNA genes, which ensures a constant balance of these two components.