Characterization of cholyl-adenylate in rat liver microsomes by liquid chromatography/electrospray ionization-mass spectrometry.

@article{Ikegawa1999CharacterizationOC,
  title={Characterization of cholyl-adenylate in rat liver microsomes by liquid chromatography/electrospray ionization-mass spectrometry.},
  author={Shigeo Ikegawa and Hayato Ishikawa and Hiroyuki Oiwa and Michiyo Nagata and Junichi Goto and Tamotsu Kozaki and M Gotowda and Naoki Asakawa},
  journal={Analytical biochemistry},
  year={1999},
  volume={266 1},
  pages={125-32}
}
The cholyl-adenylate, covalently bound 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholanoic acid (cholic acid) with adenosine 5'-monophosphate having an acid anhydride linkage, has been characterized by means of liquid chromatography/mass spectrometry in an incubation mixture with a rat liver microsomal fraction. The authentic specimen of cholyl-adenylate was synthesized using the carbodiimide method and the structure was confirmed by MS and nuclear magnetic resonance spectroscopy. After… CONTINUE READING
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