Characterization of cholecystokinin receptors on bovine gallbladder membranes.

@article{Steigerwalt1984CharacterizationOC,
  title={Characterization of cholecystokinin receptors on bovine gallbladder membranes.},
  author={R W Steigerwalt and Ira D Goldfine and John A. Williams},
  journal={The American journal of physiology},
  year={1984},
  volume={247 6 Pt 1},
  pages={G709-14}
}
Cholecystokinin (CCK) binding to its receptors on a muscularis membrane fraction of bovine gallbladder was characterized using a biologically active CCK-33-[125I]Bolton-Hunter conjugate. Receptor binding was localized to the muscularis layer of the gallbladder; no binding was seen on either mucosal or serosal membranes. At 24 degrees C and pH 6.5, binding was maximal after 60-90 min of incubation, remained at a plateau for at least 240 min, and was reversed by the addition of unlabeled CCK-8… CONTINUE READING
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