Characterization of chitin synthase 2 of Saccharomyces cerevisiae. Implication of two highly conserved domains as possible catalytic sites.

@article{Nagahashi1995CharacterizationOC,
  title={Characterization of chitin synthase 2 of Saccharomyces cerevisiae. Implication of two highly conserved domains as possible catalytic sites.},
  author={S Nagahashi and Masayuki Sudoh and Nobukazu Ono and Rumi Sawada and Emi Yamaguchi and Yoshikazu Uchida and Toshiyuki Mio and Masahiro Takagi and Mikio Arisawa and Hisafumi Yamada-Okabe},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 23},
  pages={13961-7}
}
Chitin synthase 2 of Saccharomyces cerevisiae was characterized by means of site-directed mutagenesis and subsequent expression of the mutant enzymes in yeast cells. Chitin synthase 2 shares a region whose sequence is highly conserved in all chitin synthases. Substitutions of conserved amino acids in this region with alanine (alanine scanning) identified… CONTINUE READING