Characterization of calcium ion sensitive region for β-mannanase from Streptomyces thermolilacinus.

@article{Kumagai2011CharacterizationOC,
  title={Characterization of calcium ion sensitive region for β-mannanase from Streptomyces thermolilacinus.},
  author={Yuya Kumagai and Hirokazu Usuki and Yukihiro Yamamoto and Akihiro Yamasato and Jiro Arima and Takafumi Mukaihara and Tadashi Hatanaka},
  journal={Biochimica et biophysica acta},
  year={2011},
  volume={1814 9},
  pages={1127-33}
}
Despite the widespread industrial applications of β-mannanase, the relations between the enzymatic properties and metal ions remain poorly understood. To elucidate the effects of metal ions on β-mannanase, thermal stability and hydrolysis activity were characterized. The stman and tfman genes encoding β-mannanase (EC.3.2.1.78) from Streptomyces thermolilacinus NBRC14274 and Thermobifida fusca NBRC14071 were cloned and expressed in Escherichia coli. The thermal stability of each enzyme shifted… CONTINUE READING

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