Characterization of cDNAs differentially expressed in roots of tobacco (Nicotiana tabacum cv Burley 21) during the early stages of alkaloid biosynthesis.

@article{Wang2000CharacterizationOC,
  title={Characterization of cDNAs differentially expressed in roots of tobacco (Nicotiana tabacum cv Burley 21) during the early stages of alkaloid biosynthesis.},
  author={Wang and Sheehan and Brookman and Timko},
  journal={Plant science : an international journal of experimental plant biology},
  year={2000},
  volume={158 1-2},
  pages={19-32}
}
  • Wang, Sheehan, +1 author Timko
  • Published 2000 in
    Plant science : an international journal of…
A set of 60 cDNAs were isolated by subtractive hybridization screening of a phage library using radioactively-labeled probes generated from root mRNAs isolated from tobacco (Nicotiana tabacum cv Burley 21) plants before and 3 days after topping. Among the differentially expressed gene products were full-length and partial cDNAs encoding arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and S-adenosylmethionine synthetase (SAMS), enzymes involved in polyamine and alkaloid biosynthesis… CONTINUE READING