Characterization of cDNA for precursor of human luteinizing hormone releasing hormone

  title={Characterization of cDNA for precursor of human luteinizing hormone releasing hormone},
  author={P. H. Seeburg and John P. Adelman},
Human reproduction is controlled by the hypothalamic–pituitary–gonadal axis laid down early in fetal development. Luteinizing hormone releasing hormone (LHRH), also termed gonadotropin releasing hormone (GnRH), is a decapeptide1,2 and is a key molecule in this control circuit. It is produced by hypothalamic neurones, secreted in a pulsatile manner into the capillary plexus of the median eminence and effects the release of luteinizing hormone and follicle stimulating hormone from gonadotropic… 
Evolutionary aspects of gonadotropin-releasing hormone and its receptor
Evidence suggests that in most species the early-evolved and highly conserved chicken GnRH II has a neurotransmitter function, while the second form, which varies across classes, has a physiologic role in regulating gonadotropin release.
Gonadotropin-Releasing Hormone: Differentiation of Structure and Function During Evolution
It is now clear that GnRH is a member of a family of homologous peptides, has multiple functions, is located both within and out of the central nervous system and has well-studied neuro-transmitter actions.
Origin of mammalian gonadotropin-releasing hormones.
Studying of GnRH peptides and their genes have altered views on the origin, function, and regulation of this neuropeptide, and novel functions, in addition to the release of gonadotropins exist.
Regulation of gonadotropin-releasing hormone (GnRH) gene expression in hypothalamic neuronal cells
The recent availability of immortalized neuronal cell lines that produce GnRH allows investigators for the first time to begin to dissect the factors that directly regulate GnRH gene expression.


Cloning and sequence analysis of cDNA for ovine corticotropin-releasing factor precursor
Comparison of the amino acid sequence of prepro-CRF with those of the ACTH–β-LPH precursor and the arginine vasopressin–neurophysin II precursor suggests that these precursor proteins may be evolutionarily related.
Gonadocrinins: Peptides in Ovarian Follicular Fluid Stimulating the Secretion of Pituitary Gonadotropins*
A newly discovered small peptide purified from rat follicular fluid stimulates the pituitary to release FSH and LH in vitro as well as in vivo, and is named gonadocrinin for operational facility.
Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factor, somatocrinin.
In vitro translation of tumor poly(A)+ RNA followed by immunoprecipitation with GRF-specific antiserum and gel electrophoresis showed the molecular weight of preprosomatocrinin to be approximately 13,000, which is in good agreement with the Molecular weight deduced from the sequences of the cDNA clones.
Characterization of a teleost gonadotropin-releasing hormone.
A peptide that is recognized by certain antibodies raised against mammalian gonadotropin-releasing hormone has been purified from extracts of salmon brains by gel filtration and high-performance liquid chromatography and demonstrates that most of the molecule has been conserved during evolution.
Isolation and properties of the FSH and LH-releasing hormone.
A high molecular weight precursor of luteinizing hormone releasing hormone from rat hypothalamus.
A poly A+ enriched fraction of total RNA extracted from rat hypothalamus was translated in a messenger RNA-dependent cell free system from rabbit reticulocyte lysate supplemented with 3H-leucine, suggesting the presence of a high molecular weight precursor for the decapeptide, LHRH.
Placental luteinizing hormone-releasing factor and its synthesis.
The synthesis of a placental luteinizing hormone-releasing factor (pLRF), which is immunologically, physiochemically, and biologically indistinguishable from synthetic LRF, was demonstrated and data indicate that the pLRF that was released initially was endogenous, whereas that released subsequently reflected synthesis.