Characterization of cDNA for precursor of human luteinizing hormone releasing hormone

  title={Characterization of cDNA for precursor of human luteinizing hormone releasing hormone},
  author={P. H. Seeburg and John P. Adelman},
Human reproduction is controlled by the hypothalamic–pituitary–gonadal axis laid down early in fetal development. Luteinizing hormone releasing hormone (LHRH), also termed gonadotropin releasing hormone (GnRH), is a decapeptide1,2 and is a key molecule in this control circuit. It is produced by hypothalamic neurones, secreted in a pulsatile manner into the capillary plexus of the median eminence and effects the release of luteinizing hormone and follicle stimulating hormone from gonadotropic… 
Gonadotropin-Releasing Hormone: Differentiation of Structure and Function During Evolution
It is now clear that GnRH is a member of a family of homologous peptides, has multiple functions, is located both within and out of the central nervous system and has well-studied neuro-transmitter actions.
Origin of mammalian gonadotropin-releasing hormones.
Studying of GnRH peptides and their genes have altered views on the origin, function, and regulation of this neuropeptide, and novel functions, in addition to the release of gonadotropins exist.
Regulation of gonadotropin-releasing hormone (GnRH) gene expression in hypothalamic neuronal cells
The recent availability of immortalized neuronal cell lines that produce GnRH allows investigators for the first time to begin to dissect the factors that directly regulate GnRH gene expression.
The hypothalamus-pituitary-gonad axis: Tales of mice and men.
Biosynthesis of LHRH
Homeostasis of this system, as other endocrine systems, is maintained by controlling mechanisms, and the action of hypotnalamic releasing and release-inhibiting hormones can be controlled through their biosynthesis, processing and secretion as well as at their target, the pituitary gland.


Cloning and sequence analysis of cDNA for ovine corticotropin-releasing factor precursor
Comparison of the amino acid sequence of prepro-CRF with those of the ACTH–β-LPH precursor and the arginine vasopressin–neurophysin II precursor suggests that these precursor proteins may be evolutionarily related.
Gonadocrinins: Peptides in Ovarian Follicular Fluid Stimulating the Secretion of Pituitary Gonadotropins*
A newly discovered small peptide purified from rat follicular fluid stimulates the pituitary to release FSH and LH in vitro as well as in vivo, and is named gonadocrinin for operational facility.
Characterization of a teleost gonadotropin-releasing hormone.
A peptide that is recognized by certain antibodies raised against mammalian gonadotropin-releasing hormone has been purified from extracts of salmon brains by gel filtration and high-performance liquid chromatography and demonstrates that most of the molecule has been conserved during evolution.
Isolation and properties of the FSH and LH-releasing hormone.
A high molecular weight precursor of luteinizing hormone releasing hormone from rat hypothalamus.
A poly A+ enriched fraction of total RNA extracted from rat hypothalamus was translated in a messenger RNA-dependent cell free system from rabbit reticulocyte lysate supplemented with 3H-leucine, suggesting the presence of a high molecular weight precursor for the decapeptide, LHRH.
Partial isolation and characterization of testicular GnRH-like factors.
It is concluded that there are at least two factors in rat testis with GnRH-receptor-binding properties that are chemically distinct from the native decapeptide.
Primary structure of the ovine hypothalamic luteinizing hormone-releasing factor (LRF) (LH-hypothalamus-LRF-gas chromatography-mass spectrometry-decapeptide-Edman degradation).
The primary structure of ovine hypothalamic hypophysiotropic luteinizing hormone-releasing factor, LRF, has been established as pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH(2) by hydrolysis of the