Characterization of beta-endorphin immunoreactive peptides in rat pituitary and brain by coupled gel and reversed-phase high-performance liquid chromatography.

@article{Dennis1983CharacterizationOB,
  title={Characterization of beta-endorphin immunoreactive peptides in rat pituitary and brain by coupled gel and reversed-phase high-performance liquid chromatography.},
  author={Megan Dennis and Claude Lazure and Nabil G Seidah and Michel Chr{\'e}tien},
  journal={Journal of chromatography},
  year={1983},
  volume={266},
  pages={163-72}
}
A procedure which combines gel high-performance liquid chromatography (HPLC) and reversed-phase HPLC in two systems with radioimmunoassay was developed for the analysis of beta-endorphin (beta END)-related peptides in rat pituitary and brain. The gel HPLC system allows rapid screening for relative amounts of precursors and products involved in beta END biosynthesis. Reversed-phase HPLC reveals minor post-translational modifications, such as acetylation, which greatly alter the biological… CONTINUE READING