Characterization of beta 2-microglobulin transcripts from two teleost species.

@article{Dixon1993CharacterizationOB,
  title={Characterization of beta 2-microglobulin transcripts from two teleost species.},
  author={Benjamin Dixon and Ren{\'e} J M Stet and S H van Erp and Bill Pohajdak},
  journal={Immunogenetics},
  year={1993},
  volume={38 1},
  pages={27-34}
}
Using degenerate primers based on published beta 2-microglobulin sequences we were able to obtain an expected 111 base pairs (bp) polymerase chain reaction (PCR) fragment from tilapia genomic DNA. The sequence of this fragment showed a high degree of similarity to mouse beta 2-microglobulin at the protein level. We used these primers in an "anchored PCR" to obtain a 213 bp PCR fragment from a carp cDNA library. This was then used to clone a full-length beta 2-microglobulin cDNA from carp. The… CONTINUE READING