Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers.

@article{Kong2010CharacterizationOB,
  title={Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers.},
  author={Lingbo Kong and Pengfei Zhang and Peter Setlow and Yong-qing Li},
  journal={Analytical chemistry},
  year={2010},
  volume={82 9},
  pages={3840-7}
}
We present a methodology that combines external phase contrast microscopy, Raman spectroscopy, and optical tweezers to monitor a variety of changes during the germination of single Bacillus cereus spores in both nutrient (l-alanine) and non-nutrient (Ca-dipicolinic acid (DPA)) germinants with a temporal resolution of approximately 2 s. Phase contrast microscopy assesses changes in refractility of individual spores during germination, while Raman spectroscopy gives information on changes in… CONTINUE READING
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