Characterization of anf genes specific for the alternative nitrogenase and identification of nif genes required for both nitrogenases in Rhodobacter capsulatus

@article{Schddekopf1993CharacterizationOA,
  title={Characterization of anf genes specific for the alternative nitrogenase and identification of nif genes required for both nitrogenases in Rhodobacter capsulatus},
  author={K Sch{\"u}ddekopf and Silke Hennecke and U Liese and Michael Kutsche and Werner Klipp},
  journal={Molecular Microbiology},
  year={1993},
  volume={8}
}
To identify Rhodobacter capsulatus nif genes necessary for the alternative nitrogenase, strains carrying defined mutations in 32 genes and open reading frames of nif region A, B or C were constructed. The ability of these mutants to grow on nitrogen‐free medium with molybdenum (Nif phenotype) or in a nifHDK deletion background on medium without molybdenum (Anf phenotype) was tested. Nine nif genes and nif‐associated coding regions are absolutely essential for the alternative nitrogenase. These… Expand
Functional Analysis of Multiple nifB Genes of Paenibacillus Strains in Synthesis of Mo-, Fe- and V-Nitrogenase
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TLDR
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Rhodobacter capsulatus AnfA is essential for production of Fe‐nitrogenase proteins but dispensable for cofactor biosynthesis and electron supply
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Ch Chromosomal replacement of the anfH promoter by the nifH promoter restored anfHDGK expression and Fe‐nitrogenase activity in an R. capsulatus strain lacking AnfA suggesting that An fA is required for AnfHDK production, but dispensable for biosynthesis of the iron‐only cofactor and electron delivery to Fe‐ Nitrogenase, pathways activated by NifA. Expand
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References

SHOWING 1-10 OF 62 REFERENCES
Two nifA-like genes required for expression of alternative nitrogenases by Azotobacter vinelandii
TLDR
Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii and growth data indicate that n ifA is required for a factor (or factors) necessary for maximal diazotrophic growth under Mo- and V-deficient conditions. Expand
Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region
Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomalExpand
Nucleotide sequence and genetic analysis of the Rhodobacter capsulatus ORF6-nifUI SVW gene region: possible role of NifW in homocitrate processing.
TLDR
Analysis of defined insertion and deletion mutants demonstrated that only nifS was absolutely essential for nitrogen fixation in R. capsulatus, indicating a possible role of NifW either in homocitrate transport or in the incorporation of this compound into the iron-molybdenum cofactor of nitrogenase. Expand
Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae
TLDR
The NifQ- mutants of Klebsiella pneumoniae are defective in nitrogen fixation due to an elevated requirement for molybdenum, and the nifQ gene product acts together with the products of nifB, nifN, and nifE in the biosynthesis of the iron-molyBdenum cofactor of nitrogenase. Expand
Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii
TLDR
Results indicate that anfH, anfD, and anfK encode structural proteins for nitrogenase 3 in Azotobacter vinelandii. Expand
Characterization of nif regulatory genes in Rhodopseudomonas capsulata using lac gene fusions.
TLDR
Translational fusions of the Escherichia coli lacZYA operon to Rhodopseudomonas capsulata nif genes were obtained by using mini-MudII1734 inserts into cloned fragments of R. Capsulata DNA. Expand
Demonstration of a molybdenum- and vanadium-independent nitrogenase in a nifHDK-deletion mutant of Rhodobacter capsulatus.
TLDR
The results indicate that the alternative nitrogenase of R. capsulatus is not a vanadium enzyme but rather a heterometal-free Fe-nitrogenase or a nitrogenase with an as-yet-unidentified heterometAL atom. Expand
Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus
TLDR
DNA sequence analysis of a 1,600-base-pair fragment located downstream of nifENX in nif region A of Rhodobacter capsulatus revealed two additional open reading frames (ORFs): ORF5, encoding a ferredoxinlike protein, and nifQ, and it was demonstrated that the gene encoding the ferred toxinlike protein is not essential for nitrogen fixation. Expand
Organization and regulation of nitrogen fixation genes in Rhodobacter capsulatus
The analysis of nitrogen fixation genes (nif) was first carried out in the facultative anaerobe Klebsiella pneumoniae and this organism has become a paradigm. Within the last few years theExpand
Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii
TLDR
Growth studies and acetylene reduction assays performed on two nifEN deletion strains showed that nifE and nifN are required for N2 fixation under Mo sufficiency, as previously observed, but not under conditions of Mo deficiency or in the presence of 50 nM V2O5. Expand
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