Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.

  title={Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.},
  author={Daniela Monti and Giacomo Carrea and Sergio Riva and E Baldaro and Guilherme Fernando Frare},
  journal={Biotechnology and bioengineering},
  volume={70 2},
A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optimal at pH between 7 and 9.5 and to increase with temperature and in buffered solutions. To avoid product degradation, optimal reaction conditions were obtained working at 25 degrees C using a 50-mM… CONTINUE READING


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