Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy.

@article{Day2008CharacterizationOA,
  title={Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy.},
  author={Richard N. Day and Cynthia F Booker and Ammasi Periasamy},
  journal={Journal of biomedical optics},
  year={2008},
  volume={13 3},
  pages={031203}
}
The genetically encoded fluorescent proteins (FP), used in combination with Forster resonance energy transfer (FRET) microscopy, provide the tools necessary for the direct visualization of protein interactions inside living cells. Typically, the Cerulean and Venus variants of the cyan and yellow FPs are used for FRET studies, but there are limitations to their use. Here, Cerulean and the newly developed monomeric Teal FP (mTFP) are compared as FRET donors for Venus using spectral and… CONTINUE READING

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